(classical old)chinese literature Essay Example | Topics and Well Written Essays - 1750 words

(classical old)chinese literature - Essay Example The title of the â€Å"Written Crossing the Yellow River† sets the place as Yellow River and signals the upcoming stream of pictorial images. Indeed, both the noun â€Å"river† and the verb â€Å"to cross† denote a motion and imply that the narrator sets out on a voyage. The first line begins with a noun and the narrator speaks about a define boat: the boat that he is in. The river was described as â€Å"great†. The reason of its greatness is explained in the second line as to its â€Å"swollen waters† (386). It can be inferred from the second line that the weather is either extremely bad or the narrator is exaggerating the weather conditions since he describes the waters as â€Å"stretched to the sky edge† (386). We understand from the third line that the storm has just broke out. The motion is intensified and accelerated as â€Å"the sky and waves split apart suddenly†. Although it is the boat that wavered back and forth with the wav es, the narrator perceives it as if sky and waves split apart. The waves which obscured the narrator's view, now allow him to see his hometown as we can infer from the forth line. â€Å"The thousands of homes† in his town appear momentarily with the ebb of the tides. In the fifth line, he shifts his focus from homes to the marketplace. The sixth line paints a bit blurry picture, since the narrator's actual perception and his imagination seems to be merged. The adverb â€Å"vaguely† signifies the blurring of perception and imagination as he contemplates mulberry and hemp displayed at the town's marketplace. Indeed, it was impossible for him to discern mulberry and hemp from that distance (if we accept the previous information that he sees the thousands of homes in that distance). However, he sees the mulberry and hemp with his mind eye or â€Å"makes them out†. The one and only symbol of the poem appears when his mind shifts from the actual view to the landscape of his imagination. Indeed, the symbol of â€Å"mulberry and hemp† traditionally stands for China and its cultivated lands. After a momentary distraction, in the seventh line, he brings back his focus to his homeland and fixes his gaze, nevertheless it was too late. While he turned to gaze back, the town has already been disappeared with the flow of the tides. The last line brings us back to the beginning as the waters stretched to the clouds obscuring the subject's view. The last line is metaphorically linked to the second line as the verb â€Å"stretched† repeated twice in the poem. Meanwhile, â€Å"the vast floods† replaced â€Å"swollen waters† and â€Å"the clouds† substituted for â€Å"sky's edge†. The â€Å"vast floods† and â€Å"swollen waters† function like metaphorical curtains first covering then under-covering then covering again the view of the subject. The poet uses the ebb and flow of the tides to create an optic al illusion, which hides and then reveals his homeland. This rhythmic flow of waters reminds the fort-da (Gone! and There!) game. According to Freud, the child plays this game in order to appease his anxiety due to the absence of the mother. In this case, it is the homeland that first â€Å"Gone!† then â€Å"There† again† and the subject tries to overcome his feelings of loss while he leaves his hometown behind. In fact, the rhythmic

Molecular Microbiology Case Study Example | Topics and Well Written Essays - 1500 words

Molecular Microbiology - Case Study Example The commoner intravenous catheter-related infections are exit-site infections, as in this case, often with erythema around the area where the line penetrates the skin. Bacterial blood stream infections are common in this given scenario, and Staphylococci are the second most prevalent bacteria. However, a smear suggesting the staphylococci or Gram-positive cocci in clusters in blood culture as in here, is not sufficient for the diagnosis of true bacterial blood stream infection before the species is identifiable, since the most frequent of this species, Coagulase-negative staphylococci or CoNS usually habituate in the skin, and there is always a chance of contamination of the culture bottles during the venipuncture. In contrast, such an infection due to Staphylococcus aureus is virulent by its intrinsic nature, and isolation in one blood culture bottle is clearly diagnostic and is an indication of initiation of antibiotic therapy. Thus the therapeutic decision making is based on identification of the organism (Beekmann, S. E., Diekema, D. J. and Doern, D. J., 2005). The first test obviously would be to do a light microscopic examination. Direct microscopic examination may provide a rapid, presumptive report of Gram-positive cocci resembling staphylococci. Isolation of S. aureus should be performed using 5% blood agar following an incubation period of 18-24 h in air at 35-37 C. Staphylococcus aureus ferments mannitol, resulting in a change in the colour of the medium from pink to yellow. Colony morphology may be used by the experienced observer to define presumptive staphylococci. A Gram stain appearance of cocci in clusters and a positive catalase test provide rapid indicators of staphylococci. However, in order to be able to distinguish between Staphylococcus aureus and the remaining members of the staphylococcus species, other tests are necessary. For clinical microbiological purposes, two or three simple tests suffice. The coagulase test detects the production of coagulase by S. aureus. In this test, one colony is mixed with plasma, incubate d at 37 C for 4 h and observed for clot formation. Samples that are negative at 4 h are incubated and observed again for clotting at 24 h. The slide agglutination test detects clumping factor (ClfA). This is performed by making a heavy homogenous suspension of cells in distilled water on a glass slide to which a drop of plasma is added. Within 10 s, the mixture is examined for presence of clumping (Chapin, K., and M. Musgnug, 2003). How would you differentiate the cocci in clusters from those in chain Cocci in clusters are named as staphylococci. All staphylococci have the ability to convert hydrogen peroxide into nontoxic H2O and O2. Both coagulase positive and negative staphylococci produce catalase. This test differentiates them from cocci in chain or streptococci, which cannot produce catalase and hence are catalase negative (Chapin, K., and M. Musgnug, 2003). What is the principle of DNase test and what is the identity of this organism and why Support your answer with microbiological diagnostic facts. DNase or deoxyribonuclease is an extracellular enzyme that can hydrolyze deoxyribonucleic acid to oligonucleotides. Several varieties of deoxyribonucleases are distinguished on the basis of antigenic properties, response to inhibitory substances, hydrolytic end products, and